3,6-diamino-9-[2-(methoxycarbonyl)phenyl]xanthenium chloride
3,6-diamino-9-[2-(methoxycarbonyl)phenyl]xanthylium chloride
CAS: 62669-70-9
Molecular Formula: C21H17ClN2O3
3,6-diamino-9-[2-(methoxycarbonyl)phenyl]xanthenium chloride - Names and Identifiers
| Name | 3,6-diamino-9-[2-(methoxycarbonyl)phenyl]xanthylium chloride |
| Synonyms | RH 123 Rhodamine 123 Rhodamine 123, pure Rhodamin 123 Chloride, laser grade Rhodamine methyl ester hydrochloride 3,6-diamino-9-[2-(methoxycarbonyl)phenyl]xanthenium chloride 3,6-diamino-9-[2-(methoxycarbonyl)phenyl]xanthylium chloride 3,6-Diamino-9-(2-(methoxycarbonyl)phenyl)xanthylium chloride Xanthylium, 3,6-diamino-9-(2-(methoxycarbonyl)phenyl)-, chloride 6-amino-9-[2-(methoxycarbonyl)phenyl]-3H-xanthen-3-iminium chloride 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester monohydrochloride Benzoic acid, 2-(6-amino-3-imino-3H-xanthen-9-yl)-, methyl ester, monohydrochloride |
| CAS | 62669-70-9 |
| EINECS | 263-687-8 |
| InChI | InChI=1/C21H17N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11H,22-23H2,1H3;1H/q+1;/p-1 |
| InChIKey | MYFATKRONKHHQL-UHFFFAOYSA-N |
3,6-diamino-9-[2-(methoxycarbonyl)phenyl]xanthenium chloride - Physico-chemical Properties
| Molecular Formula | C21H17ClN2O3 |
| Molar Mass | 380.83 |
| Density | 1.2224 (rough estimate) |
| Melting Point | 235 °C |
| Water Solubility | Partially soluble |
| Solubility | Soluble in ethanol (20 mg/ml), DMSO, methanol, and water (partly miscible). |
| Appearance | Powder |
| Color | Red-brown to brown or dark green |
| Maximum wavelength(λmax) | ['501 nm, 507 nm'] |
| BRN | 6030951 |
| Storage Condition | Hygroscopic, -20°C Freezer, Under inert atmosphere |
| Stability | Hygroscopic |
| Refractive Index | 1.6500 (estimate) |
| MDL | MFCD00012664 |
| In vitro study | The intensity of R123 fluorescence has a peak at concentration of 50 μM, and decreases to zero at higher concentrations due to self-quenching. |
3,6-diamino-9-[2-(methoxycarbonyl)phenyl]xanthenium chloride - Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | 22 - Harmful if swallowed |
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| RTECS | ZE0883000 |
| HS Code | 32049010 |
3,6-diamino-9-[2-(methoxycarbonyl)phenyl]xanthenium chloride - Reference
| Reference Show more | 1. Liu, Xue, he, Yinglan, Hu, Yueying, et al. Antibacterial Activity and mechanism of 3-camphene against Pseudomonas aeruginosa [J]. Chinese Journal of Tropical Crops, 2019, 40(03):191-198. 2. Shu Huizhen, Tang Zhiling, Liu Xue, etc. Antibacterial Activity and mechanism of limonene against Pseudomonas fluorescens [J]. Science and Technology of food industry, 2019, v.40;No.428(12):140-146. 3. Liu Xue, Wang Jingnan, Chen Wenwen, et al. Antibacterial activity of limonene against Pseudomonas aeruginosa and its mechanism [J]. Science and Technology of food industry, 2018, 039(007):1-5. 4. Yu Li, Yu zhenlan, Jia Yanli. Therapeutic effect and significance of sunitinib on gefitinib-resistant lung cancer cells [J]. Chinese Pharmaceutical Journal, 2017(03):244-248. 5. Guo Yuyu, Chen Wenwen, Chen Haiming, et al. Antibacterial mechanism of linalool on Escherichia coli [J]. Modern food science and technology, 2020(4):113-118. 6. Liu, Han, et al. "Astaxanthin attenuates d-galactose-induced brain aging in rats by ameliorating oxidative stress, mitochondrial dysfunction, and regulating metabolic markers." Food & function 11.5 (2020): 4103-4113.https://doi.org/10.1039/D0FO00633E 7. Zhai, Junqiu, et al. "Exenatide-loaded inside-porous poly (lactic-co-glycolic acid) microspheres as a long-acting drug delivery system with improved release characteristics." Drug Delivery 27.1 (2020): 1667-1675.https://doi.org/10.1080/10717544.2020.185091 8. [IF=6.419] Junqiu Zhai et al."Exenatide-loaded inside-porous poly(lactic-co-glycolic acid) microspheres as a long-acting drug delivery system with improved release characteristics."Drug Deliv. 2020;27(1):1667-1675 9. [IF=5.64] Fengyu Guo et al."Antimicrobial Activity and Proposed Action Mechanism of Linalool Against Pseudomonas fluorescens."Front Microbiol. 2021 Jan 28;12:562094 10. [IF=5.396] Han Liu et al."Astaxanthin attenuates D-galactose-induced brain aging in rats by ameliorating oxidative stress, mitochondrial dysfunction, and regulating metabolic markers."Food Funct. 2020 May;11(5):4103-4113 11. [IF=4.411] Huizhen Shu et al."Antimicrobial Activity and Proposed Action Mechanism of 3-Carene against Brochothrix thermosphacta and Pseudomonas fluorescens."Molecules. 2019 Jan;24(18):3246 12. [IF=4.411] Fengyu Guo et al."Antibacterial Activity and Mechanism of Linalool against Shewanella putrefaciens."Molecules. 2021 Jan;26(1):245 13. [IF=3.738] Xue Liu et al."Antibacterial activity and mechanism of linalool against Pseudomonas aeruginosa."Microb Pathogenesis. 2020 Apr;141:103980 14. [IF=2.863] Bin Zheng et al."10-Gingerol alleviates hypoxia/reoxygenation-induced cardiomyocyte injury through inhibition of the Wnt5a/Frizzled-2 pathway."Food Sci Nutr. 2021 Jul;9(7):3917-3931 |
3,6-diamino-9-[2-(methoxycarbonyl)phenyl]xanthenium chloride - Introduction
Rhodamine 123 (Rhodamine 123, Rhodamine 123, Rh 123) is a commonly used cationic green fluorescent dye, which can penetrate the cell membrane and accumulate in the mitochondria of living cells and emit yellow-green fluorescence. It is widely used as a fluorescent probe for detecting mitochondrial membrane potential and does not have any toxicity to cells. The solution was dissolved with DMSO at a final concentration of 1-5 mM. The solution is packed and stored at -20°C away from light. Rhodamine 123 Use Method 1. Prepare cells with glass slides. The number of cells should be 5 × 104~5 × 105 cells/mL. 2. Incubate the slide and wash the cells with PBS or Hank's solution. 3. Dilute Rh123 mother liquor with culture medium to prepare 1~20 µM Rh123 buffer. The specific working concentration depends on the cell type and cell concentration. 4. Add Rh123 buffer to the slide and incubate at 37°C for 30 minutes to 1 hour. 5. Remove Rh123 buffer and wash the cells with culture medium (after washing the cells, for fixation, add 10% formalin buffer and incubate for 15-20 minutes, then wash with PBS). 6. The cells were observed by fluorescence microscope with fluorescein filter. For your safety and health, please wear experimental clothing and disposable gloves.
Last Update：2022-10-16 17:27:24
3,6-diamino-9-[2-(methoxycarbonyl)phenyl]xanthenium chloride - Reference Information
| Main applications | Colored prices; colorfilters; liquid crystal displays; light-emitting diodes; Luminal materials;nanophotonic composites;paints; semiconductor electrodes;solar cells |
| Biological applications | Measuring membrane potential; detecting cancer cells,spores,prostate cancer,stress biomarkers; treating disc degenerative disease,epilepsy, ereclide dysfunction; Apoptosises; tumor cell multidrug resistance assay; Table |
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| biological activity | Rhodamine 123 (RH-123; R-22420) is a fluorescent dye (λex = 503 nm, λem = 527 nm). |
| Use | is a selective staining that can penetrate cell membranes. |
Last Update：2024-04-09 20:45:29
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